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2024 Vol.1, Issue 1

Research Article

A tufA reference sequence library for the green algal genus Caulerpa (Bryopsidales)
Stefano G.A. Draisma1*
,
Thomas Sauvage2
1Excellence Center for Biodiversity of Peninsular Thailand, Science Faculty, Prince of Songkla University, Hat Yai, 90110 Songkhla, Thailand
2Smithsonian Marine Station at Ft. Pierce, 34949 Florida, United States
*Corresponding Author
30 April 2024. pp. 1-11
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Abstract

The circumtropical, multinucleate, siphonous, green algal genus Caulerpa is the single genus in the family Caulerpaceae and exhibits a remarkably high morphological variability. Morphological plasticity, overlapping morphological species boundaries, and the occurrence of cryptic species, has led several scientists to advocate the use of DNA barcodes for reliable species identification. The chloroplast-encoded tufA gene has been the DNA barcode marker of choice for Caulerpa, in particular the 820 nucleotides central region. Molecular identification through DNA barcodes depends on the availability of a curated reference library with pre-defined species. However, currently there are many incorrect Caulerpa species names in the GenBank sequence data base, potentially causing error propagation. This complicates species identification by researchers who are not familiar with Caulerpa. The present study reviewed the published CaulerpatufA sequences, generated new tufA sequences for four species, and selected reference sequences for ¾ of the currently accepted Caulerpa species. Reference sequences were primarily selected based on geographic proximity to the type location. This resulted in a tufA sequence alignment of 89 Caulerpa taxa of which four remained unnamed. Caulerpa ambigua, C. verticillata, and C. lanuginosa represent cryptic species complexes of, respectively, nine, three, and two species. The tufA sequences of C. macra, C. manorensis, and C. veravalensis were compared for the first time and the small sequence difference suggests that these could represent a single variable species.

Keywords
Bryopsidales
Caulerpa
cryptic species
dark taxa
DNA barcoding
macroalgae
Pseudochlorodesmis
reference sequence
Siphonogramen
tufA
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Information
  • Publisher :Jeju Journal of Island Sciences
  • Publisher(Ko) :제주섬과학회지
  • Journal Title :Jeju Journal of Island Sciences
  • Journal Title(Ko) :제주섬과학회지
  • Volume : 1
  • No :1
  • Pages :1-11
  • Received Date : 2024-01-09
  • Revised Date : 2024-03-22
  • Accepted Date : 2024-03-26
  • DOI :https://doi.org/10.23264/JJIS.2024.1.1.001
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